Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi.

Conn JE, Bollback JP, Onyabe DY, Robinson TN, Wilkerson RC & Povoa MM

(2001) Mol Ecol Notes 1, 223-225.

High molecular weight DNA was extracted from the primary Neotropical malaria vector, Anopheles darlingi from Capanema, ParĂ¡, Brazil, to create a small insert genomic library, and then a phagemid library. Enriched sublibraries were constructed from the phagemid library using a microsatellite oligo primed second strand synthesis protocol. The resulting 242 760 individual clones were screened. The mean clone size of the positive clones was 302 bp. Flanking primers were designed for each suitable microsatellite sequence. Eight polymorphic loci were optimized and characterized. The allele size ranges are based on 253 samples of A. darlingi from eastern Amazonian and central Brazil.

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Andrew Rambaut, 2007